Rational design yields RNA-binding zinc finger domains with altered sequence specificity

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FIGURE 2.
FIGURE 2.

Benchmarking of the modified RBNS assay. (A–D) Fold enrichment score of all RNA 6-mers (top panel) or top 10 RNA 6-mers of ZRANB2 N′-ZnF, ZRANB2 C′-ZnF, RBM5 ZnF, and ZRANB2 C′-ZnF W79A mutant. (E) KpLogo analysis schematics. (F) KpLogo results of ZRANB2 N′-ZnF, ZRANB2 C′-ZnF, RBM5 ZnF, and ZRANB2 C′-ZnF W79A mutant.

This Article

  1. Published in Advance December 10, 2024, doi: 10.1261/rna.080329.124 RNA 31: 150-163

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