FIGURE 1.
Exploiting MCP–RNA interactions to investigate the influence of mRNA-binding proteins on gene expression. (A, top) Summary of the impact of RNA secondary structure and small molecule or protein binding upstream of the start codon, as established in prior work. (Bottom) Effects of protein binding on translation evaluated here as a function of binding affinity, size, dimerization potential, and concentration. (B) Model of the MCP-M protein–RNA complex. Location of interdomain linker and sites of mutation are emphasized. Affinities are estimated based on prior studies (Peabody 1993). (C) Architecture of reporter mRNAs.
