Integrated NMR and MD structure and dynamics of the stem–loop-II motif (s2m) from the Omicron variant of SARS-CoV-2

NMR imino proton assignment of s2m_omicron. (A) 2D-NOESY of the imino region of s2m. The chemical shift assignments of H1 and H3 imino protons of the G and U nucleotides are annotated. Sequential walks are highlighted as gray lines. (B) ¹H, ¹⁵n BEST-TROSY spectrum. H1–N1 and H3–N3 correlations are annotated. (C) ¹H, ¹⁵n HNN-COSY spectrum. Chemical shift assignments are annotated. The secondary structure of s2m_omicron is depicted on the right. Lowercase letters indicate added nucleotides for stability and transcription efficiency. All spectra were measured at 278 K.