Poly(A) tail length distribution in synaptoneurosome-derived fractions containing mRNA bound to monosomes, polysomes, and total mRNA, 10 min after in vitro NMDA-R stimulation (cDNA sequencing). (A) Polyadenylation profile of ribosomal-bound mRNAs in synaptoneurosomes fractions upon NMDA-R stimulation. P-values between conditions were calculated using Wilcoxon signed-rank test, two-sided, α = 0.05, with Benjamini–Hochberg adjustment. Dashed lines divide the plot area into sectors based on P-value cut-off points and differences in poly(A) tail lengths. Zoomed view [cut-off −log10(adj. P-value) = 10] highlighting statistically significant gene alterations. Most mitochondrial genes were omitted for clarity (high adj. P-value, unchanged tail length). Unscaled versions of these plots are provided in Supplemental Figure 10. (B) Poly(A) tail length distribution in synaptoneurosome-derived fractions containing mRNA bound to monoribosomes, polyribosomes, and total (unfractionated) mRNA, based on cDNA sequencing of selected transcript groups. (Left to right) Monoribosomal, polyribosomal, and total mRNA data. Dashed lines indicate median tail lengths for each condition, with corresponding median values shown above the plot. The total number of reads per condition (n) is also indicated.
