TRAP1 only binds cytosolic transcripts. (A) TRAP1 eCLIP density in different transcript regions. (B) Meta-analysis of TRAP1 binding around the CDS. (C) TRAP1 main binding motifs in PEA1 cells. (D) Pathway enrichment analysis of PEA1 reproducible peaks. (E) TRAP1 eCLIP profiles for selected genes. (F) Formaldehyde cross-linked RNA-IP (RIP) of TRAP1 and control IgG from PEA1 cells. qRT-PCR for LUC7L3 and four control genes. Percentage of immunoprecipitated input is shown for both TRAP1 IP and control IgG. LUC7L, LUC7L, ATP8, and PPIA were used as negative controls. Statistically significant differences were detected using two-way ANOVA and Bonferroni correction for multiple comparison testing (n = 2). Statistical significance is represented as follows: (*) P < 0.05, (**) P < 0.01, (***) P < 0.001. Error bars represent SEM.
