TRAP1 does not bind RNA through a well-defined domain. (A) Schematic representation of TRAP1 domains (ATPase, S5-D2 fold like, and C-term). Peptides retrieved from RBR-ID, pCLAP, and OOPS databases are indicated. MTS, mitochondria targeting sequence. (B) Assessment of RNA-binding of TRAP1-FLAG-HA deletion mutants in HeLa FITR eGFP cells by OOPS. hnRNPC and eGFP were used as positive and negative controls, respectively. (C,D) Assessment of RNA-binding of TRAP1 domains in HeLa FITR eGFP cells by OOPS. (Left) RNA-binding analysis of domains without the MTS sequence (n = 4). (Right) RNA-binding analysis of domains including the MTS sequence (n = 3). hnRNPC and eGFP were used as positive and negative controls, respectively. Significance was assessed by one-sample t-test. Statistical significance is represented as follows: (*) P < 0.05, (**) P < 0.01, (***) P < 0.001. Error bars represent SEM.
