Embryos produced in the oma-1 (AAA) mutant have polynucleated embryonic cells and permeable eggshells. (A) DIC images of WT and AAA mutant animals in the presence and absence of K-NAA. Example polynucleated embryos are identified with yellow arrows. The scale bar represents 20 µm. (B) Violin plots displaying the fraction of observable embryos with polynucleated cells per adult imaged. Experiments were performed as a function of genotype and growth condition. The coloring of different samples and the statistical significance are presented as in Figure 4. (C) FM4-64 treatment of embryos recovered by dissection of WT or AAA mutant animals as a function of K-NAA treatment. DIC images and FM4-64 fluorescence images are shown. The scale bar represents 20 µm. (D) Violin plots displaying the mean FM4-64 fluorescence intensity in embryos recovered from both strains and both treatment conditions. The coloring and statistical significance are as in Figure 4.
