A strain to recover and evaluate strong alleles of oma-1. (A) The genotype of the background strain used in this work (WRM66), along with a representative image showing the oocytes and embryos (DIC), the pattern of OMA-1::GFP expression (GFP), and the pattern of expression of a Tir1::mRuby transgene (mRuby) expressed in the germline and early embryos needed for auxin-mediated depletion of OMA-2 protein. The scale bar represents 20 µm. (B) Schematic of ovulation in C. elegans, including late-stage oogenesis, sperm, the oocyte-to-embryo transition (fertilization), and early embryogenesis. (C) Violin plots displaying the total and viable brood per adult hermaphrodite. Experiments were conducted in the presence and absence of control or GFP-targeting RNAi when cultured on standard nematode growth media (NGM, gray) or NGM supplemented with K-NAA (a soluble auxin salt, orange). Each dot represents the brood from a single animal. The solid black bar represents the mean of all animals measured. The dashed lines indicate statistical significance (Padj < 0.05) between culture conditions (black) or between RNAi treatments (red) in a one-way ANOVA with Bonferroni correction.
