A conserved triple arginine motif in OMA-1 is required for RNA-binding activity and embryo viability

A triple arginine motif outside of the TZF contributes to high-affinity RNA-binding. (A) Schematic of the OMA-1 variant (1–182) and the R-rich peptide. The KKK and AAA mutant variants are shown. (B) The apparent equilibrium dissociation constants (K_D,app) and Hill coefficients (n) of the binding curves with MBP-tagged OMA-1 variants shown in C are presented for three different RNA sequences. Curves for WT are in black, the KKK mutant are blue, and the AAA mutant are red. (D) The sequences used for the binding experiments are shown, and the source of the sequence is indicated to the right.