TABLE 2.
Site-directed mutagenesis primers targeting nucleotide positions 312 and 315 in PR8 segment 5
| Target strain | Segment 5 mutation | Sequence |
|---|---|---|
| PR8 WT | T312A_Fw | GTCCTCCAGTTTTCTTTGGATCTTTCCCCGCAC |
| T312A_Rev | GTGCGGGGAAAGATCCAAAGAAAACTGGAGGAC | |
| G315A_Fw | ATAGGTCCTCCAGTTTTTTTAGGATCTTTCCCCGC | |
| G315A_Rev | GCGGGGAAAGATCCTAAAAAAACTGGAGGACCTAT | |
| T312A G315A_Fw | TATAGGTCCTCCAGTTTTTTTTGGATCTTTCCCCGCACTG | |
| T312A G315A_Rev | CAGTGCGGGGAAAGATCCAAAAAAAACTGGAGGACCTATA | |
| PR8 segment 5 CpGH | A312T_Fw | GGTCCTCCAGTTTTTTTAGGATCTTTACCCGCGCT |
| A312T_Rev | AGCGCGGGTAAAGATCCTAAAAAAACTGGAGGACC | |
| A315G_Fw | ATGGGTCCTCCAGTTTTCTTTGGATCTTTACCCGC | |
| A315G_Rev | GCGGGTAAAGATCCAAAGAAAACTGGAGGACCCAT | |
| A312T A315G_Fw | AATGGGTCCTCCAGTTTTCTTAGGATCTTTACCCGCGCTC | |
| A312T A315G_Rev | GAGCGCGGGTAAAGATCCTAAGAAAACTGGAGGACCCATT |
