FIGURE 4.
Tiled reversion of the segment 5 CpGH sequence to WT identifies a short region that is common to viruses with WT virus fitness. (A) To identify the recoded region of the CpGH virus contributing the attenuated phenotype, overlapping fragments A–E were reverted to WT PR8 sequence as indicated. (B) Fragmented reversion viruses (e.g., CpGH virus with fragment A reverted to WT PR8 sequence, denoted as CpGH/A.PR8) were used to infect A549-cas9 cells at MOI 0.01 for 48 h, and virus production was measured.
