FIGURE 2.
CpG enrichment in segment 5 of the IAV genome does not impair viral packaging. (A) RNA was extracted from 107 PFU virus stocks for WT PR8, CDLR control, and CpGH viruses, and relative copy numbers of segment 1 and segment 5 RNA were quantified by qPCR. A “4c6c” packaging mutant was used as a negative control. (B) 1010 PFU of egg-derived virus panel virions were purified, then genomic RNA was extracted, and individual segments were separated using urea-PAGE and visualized by silver staining. Numbers indicate location of each genome segment. Mock is RNA from the allantoic fluid of uninfected eggs, prepared using the same methods.
