40S ribosomal subunits scan mRNA for the start codon by one-dimensional diffusion

TABLE 1.

Yeast strains used in this work

Strain Genotype Reference
BY4741 S288C MATa his3- leu2- met15- ura3-
BY4742 S288C MATα his3- leu2- lys2- ura3-
tif1Δ BY4741 tif1::hphMX This study
tif2Δ BY4741 tif2::hphMX This study
tif1Δ tif2Δ tif1-A79V (H5121-NSY21) MATa his3- leu2- ura3- met15- tif1::hphMX tif2::KanMX pSSC120 [tif1-A79V Leu2 CEN4] Sen et al. 2015
tif1Δ tif2Δ TIF1 (H5120-NSY20) MATa his3- leu2- ura3- met15- tif1::hphMX tif2::KanMX pFJZ600 [TIF1 Leu2 CEN4] Sen et al. 2015
tif3Δ BY4741 tif3::kanMX This study
tif4631Δ BY4741 tif4631::kanMX This study
tif4632Δ BY4741 tif4632::kanMX This study
tif4632Δ tif4631-L614F BY4741 tif4632::kanMX tif4631-L614F::hphMX Re-created from Park et al. 2013
tif4631Δ tif4632-M570K BY4741 tif4632::kanMX tif4631-M570K::hphMX Re-created from Park et al. 2013
tif35 K194A-L235A-F237A BY4741 tif35-K194A-L235A-F237A::KanMX Re-created from Cuchalova et al. 2010
tif34 Q258R BY4741 tif34-Q258R::KanMX Re-created from Cuchalova et al. 2010
ded1-120 (G108D/G494D) BY4741 ded1-120::KanMX Sen et al. 2015
asc1Δ BY4741 asc1::HIS5 Wolf and Grayhack 2015
slh1Δ BY4741 slh1::kanMX This study

This Article

  1. RNA 31: 1488-1502