Mutations in eIF3g and eIF3i similarly reduce translation of GFP NUS1 mRNAs with short and long unstructured 5′ UTRs. Amino acid substitutions were introduced in eIF3g-encoding tif35 (A) or eIF3i-encoding tif34 (B) in the BY4741 genetic background. GFP fluorescence measured in mutant strains was normalized to the respective GFP fluorescence measured in the wild-type (BY4741) strain. Error bars indicate standard deviations determined from three biological replicates. In experiments with the temperature-sensitive tif35-KLF strain, the Gal 1,10 promoter was induced concurrently with raising the incubation temperature from 30°C to 37°C for both tif35-KLF and wild-type cells.
