The oligonucleotides containing N7-regioisomer of guanosine: influence on thermodynamic properties and structure of RNA duplexes

TABLE 2.

Comparison of the influence of 5′- and 3′-adjacent base pairs on the thermodynamic stability of RNA duplexes containing 7G

Interacting base pairs	5′- and 3′-adjacent base pairs
	5′GC/3′CG			5′CG/3′GC			5′AU/3′UA			5′UA/3′AU
	$\text{[math]}$ ^a (kcal/mol)	$\text{[math]}$ ^b (kcal/mol)	$\text{[math]}$ ^c (kcal/mol)	$\text{[math]}$ ^a (kcal/mol)	$\text{[math]}$ ^b (kcal/mol)	$\text{[math]}$ ^c (kcal/mol)	$\text{[math]}$ ^a (kcal/mol)	$\text{[math]}$ ^b (kcal/mol)	$\text{[math]}$ ^c (kcal/mol)	$\text{[math]}$ ^a (kcal/mol)	$\text{[math]}$ ^b (kcal/mol)	$\text{[math]}$ ^c (kcal/mol)
7G-C	13.74	9.01	4.73	13.46	8.84	4.62	10.51	5.40	5.11	10.24	4.86	5.38
7G-U	11.53	8.32	3.21	11.01	9.24	1.77	7.08	5.93	1.15	6.95	3.68	3.27
7G-A	8.45	9.31	−0.86	8.03	9.44	−1.39	4.52	4.88	−0.36	7.48	3.72	3.76
7G-G	10.30	11.25	−0.95	9.72	11.48	−1.76	6.43	7.77	−1.34	7.44	7.21	0.23

^aFree energy of duplexes containing G.
^bFree energy of duplexes containing 7G.
^cFree energies ( $\text{[math]}$ ) calculated with MeltWin 3.5, using 1/T_M versus log C_T plots, were used to compare the thermodynamic stability of duplexes.

This Article

Published in Advance October 28, 2024, doi: 10.1261/rna.080106.124 RNA 2025. 31: 86-99

Current Issue

July 2026, 32 (7)