The oligonucleotides containing N7-regioisomer of guanosine: influence on thermodynamic properties and structure of RNA duplexes

The imino proton regions of the ¹H NMR spectra of the studied duplexes. (A) The 7G-A mismatch in 5′-G7GC/5′-GAC sequential context, (B) the 7G-A mismatch in 5′-C7GG/5′-CAG sequential context, (C) the 7G-G mismatch in 5′-G7GC/5′-GGC sequential context, (D) the 7G-G mismatch in 5′-C7GG/5′-CGG sequential context. All spectra were recorded at 15°C. For both 7G-G containing duplexes, a second, minor spectral form was present; its imino resonances are marked in red stars.