(A) A phylogenetic tree composed of 1573 Csps, visualizing amino acid residues at certain positions. The detailed method is described in Materials and Methods. The Csps that were used as queries for the PSI-BLAST search are shown; two Salmonella Csps (GCF_000210855.2: CBW17860.1, CBW16717.1) were not indicated because they were not included in the RefSeq database (October 2022 data set). Inner ring: proteins that contain K or D at the same position as EcCspD K43 are represented by red or green, respectively. Outer ring: proteins that contain A or E at the same position as EcCspD A44 are represented by red or green, respectively. Clusters of K-A and K-E types are indicated in orange and purple, respectively. (B) Distribution of K-A and K-E types in bacterial genomes. A phylogenetic tree was constructed from the bacterial genomes, in which one or more genes encoding the K-A type and K-E type are present. Each type of Csp was mapped to the owner genomes. Bacterial phyla and classes are shown to the left. Horizontal axes represent the number of each type of the contained Csps. The detailed method is described in Materials and Methods.
