Loss of EBAX-1 causes increased repression of miR-35–42 target mRNAs. (A) The effect of EBAX-1 on mRNA levels in L1 larvae. Plotted are fold changes in mean mRNA levels (from two biological replicates) observed upon loss of EBAX-1 in L1 larvae. The mRNA levels are expressed as counts per million mRNA reads (CPM) and filtered for those with mean wild-type expression level ≥5 CPM. Points for differentially regulated mRNAs meeting a DESeq2 adjusted P-value threshold of <0.05 are colored in red. (B) The influence of EBAX-1 on the expression of predicted mRNA targets of miR-35–42 in L1 larvae. Plotted are cumulative distributions of mean mRNA fold changes observed upon loss of EBAX-1, comparing the effect on predicted targets (red, left), predicted conserved targets (red, right), or control mRNAs lacking sites to miR-35–42 (blue). Statistical significance of differences between fold-change distributions was evaluated using the Kolmogorov–Smirnov (K–S) test.
