EBAX-1 is required to clear miR-35–42 at the embryo-to-L1 transition. (A) Sequence alignment of the eight members of the miR-35 family. (B,C) The effect of EBAX-1 on miR-35–42 levels across development. Expression of miR-35–42 family members in wild-type worms at each stage of development is shown in gray. Expression in ebax-1(tm2321) worms is plotted in red. Values for each of two replicates are plotted as separate points, whereas lines connect means of replicates. Expression of miR-42 is plotted separately as this miRNA is produced from a different primary transcript than that of miR-35–41. (D) The influence of EBAX-1 on miR-35 levels. Shown is an RNA blot comparing miR-35 and miR-52 levels in wild-type and ebax-1 mutant worms at the stages indicated. Levels of miR-35 were normalized to those of miR-52 and then to levels of EE (%EE). Levels in ebax-1 were also normalized to those of WT (ebax-1/WT).
