Discrete measurements of RNA polymerase and reverse transcriptase fidelity reveal evolutionary tuning

TABLE 2.

Error rates (×10−6) for cDNA and second-strand synthesis

Enzyme Synthesis Substitution Deletion Insertion Total
Induro cDNA 57 ± 3 6 ± 1 49 ± 4 113 ± 2
Second strand 37 ± 5 28 ± 4 52 ± 2 117 ± 10
L1-RT cDNA 220 ± 4 9 ± 0 49 ± 2 278 ± 6
Second strand 567 ± 40 15 ± 4 132 ± 5 714 ± 47
FBu cDNA 117 ± 67 7 ± 1 52 ± 12 175 ± 62
Second strand 101 ± 15 18 ± 3 73 ± 8 192 ± 22

This Article

  1. Published in Advance June 28, 2024, doi: 10.1261/rna.080002.124 RNA 30: 1246-1258

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