Discrete measurements of RNA polymerase and reverse transcriptase fidelity reveal evolutionary tuning

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

Substitution error spectrum for RNA polymerase and cDNA synthesis by RT enzymes. Unless noted, the template sequence used for fidelity measurement is DNA-1 (Supplemental Table S1). Substitutions key shows RNA polymerase errors. Errors for reverse transcriptases occur for the deoxyribose form of the base with uracil being replaced by thymine.

This Article

  1. Published in Advance June 28, 2024, doi: 10.1261/rna.080002.124 RNA 30: 1246-1258

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA