TABLE 2.
ddPCR primers/probes
| Primer name | Sequence |
|---|---|
| CBh FW (oJM358) | 5′-ACGCCAATAGGGACTTTCCAT-3′ |
| CBh RV (oJM359) | 5′-ACGTCAATAGGGGGCGTACT-3′ |
| GeFo3 FW (oJM351) | 5'-CCGGACTTGTCATGATCTCC-3' |
| GeFo3 RV (oJM285) | 5'-CCGGGTTAAGTAAAGGTGGC-3' |
| RPP30 FW (oJM366) | 5'-GATTTGGACCTGCGAGCG-3' |
| RPP30 RV (oJM367) | 5'-GCGGCTGTCTCCACAAGT-3' |
| Probe name | Sequence |
|---|---|
| CBh (prJM005) | 5′-/56-FAM/ACGGTAAAC/ZEN/TGCCCACTTGGCAGTACA/3IABkFQ/-3′ |
| GeFo3 (prJM001) | 5'-/56-FAM/CCGTCCACC/ZEN/TTGGACAAGTCTG/3IABkFQ/-3' |
| RPP30 (prJM011) | 5'-/5HEX/CTGACCTGA/ZEN/AGGCTCTGCGCG/3IABkFQ/-3' |
