Designed HDV-like Rzs confer efficient RNA template 5′ module function. (A) rRNA lengths of designed Rz-28 HDV-like Rzs, aligned to upstream rDNA sequence; −28 indicates the cleavage position in the rRNA sequence, and values in parentheses indicate the number of nucleotides of rRNA sequence included. (B) Analysis of Rz-28 cleavage from L8 leader. Reaction products were separated by 12% Urea-PAGE. (C) Quantification of insertion efficiency for RNA templates with the indicated Rz-28 5′ modules as determined by flow cytometry. Values indicate mean ± SD, n = 3. (D) PCR amplification of 3′ (top) and 5′ (bottom) rDNA-transgene junctions from gDNA in a representative replicate of the experiment in C.
