TABLE 1.
Alleles affecting transitions between the two catalytic conformations of the spliceosome
| Name | Allele | Source | |
|---|---|---|---|
| First-step catalytic conformation | prp16-302 | R456K and G691R | Madhani and Guthrie 1994 |
| prp16-R686l | R686l | Hotz and Schwer 1998 | |
| U6-lower ISL | G63c | Eysmont et al. 2019 | |
| Transition-state suppressors of prp16-302 | U6-upper ISL | A79g | Eysmont et al. 2019 |
| U6-catalytic triplex | A59c, C61a | Eysmont et al. 2019 | |
| U6-upstream of ACAGA | ΔA41, A41c, A41g, A42c, A42g, ΔC43, C43g, C43u, ΔA44, A44c, A44u, A45g, U46a, U46g | Madhani and Guthrie 1994; this study | |
| cwc2 | W37A | Hogg et al. 2014 | |
| E17D, S38P, Q54L, P55S, Q56H, D98V, K101T, C111R, l127T, N134S, F162l, W225R | This study | ||
| prp8 N-terminal | D278G, F331L, Y356C, T589P, Y590F, Y590C, K603l, K611L, K611l, K611T, K611E, S613T | This study | |
| Second-step catalytic conformation | U6-lower ISL | G63c | Eysmont et al. 2019 |
| prp8 second step | V1870N, W1575R | Liu et al. 2007 |
