Modeling the structure and DAP5-binding site of the FGF-9 5′-UTR RNA utilized in cap-independent translation

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 3.
FIGURE 3.

FGF-9 5′ UTR–DAP5 toeprinting data. FGF-9 5′-UTR cDNA was reverse-transcribed from DAP5-bound mRNA. n = 4. Higher peak area corresponds to a higher proportion of RT stops in the presence of DAP5. Size standard peaks visible at 20, 40, 50, 60, 70, 80, 100, 120, 140, 160, 170, and 180 nt. Strong RT stop at nt 93 (black arrow). Start codon: nts184–186 (magenta arrow).

This Article

  1. Published in Advance June 12, 2024, doi: 10.1261/rna.080013.124 RNA 30: 1184-1198

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA