FIGURE 5.
Chemical destabilization of 3′X55. Representative transfer efficiency histograms of 3′X55 under various destabilizing conditions show that the addition of urea to baseline experimental conditions (light to dark violet) shifts the 〈E〉 of both populations to lower values and increases the value of ϕint. When NaCl and MgCl2 are excluded from the destabilizing measurements, 3′X55 unfolds and samples a substantially more expanded low-transfer efficiency population (orange).
