Adenosine modifications impede SARS-CoV-2 RNA-dependent RNA transcription

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FIGURE 2.
FIGURE 2.

Pseudo-first-order kinetics of SARS-CoV-2 transcription shows that modified RNA templates decrease the rate of nucleotide addition. (A–C) K1/2 curves constructed by determining the kobs for single-nucleotide addition using RNA templates with adenosine (A), m6A (B), or Am (C) in the +1 position over a range of UTP concentrations. (D) kmax values for each template calculated from K1/2 curve fit. (E) K1/2 values for each template calculated from K1/2 curve fits. (F) Representative time courses for single-nucleotide addition reactions using templates with A, m6A, or Am in the +1 position. *The half-time for UTP addition on the unmodified template is lower than the dead time of the quench flow apparatus; therefore, kmax and K1/2 represent minimum limits.

This Article

  1. Published in Advance June 28, 2024, doi: 10.1261/rna.079991.124 RNA 30: 1141-1150

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