Adenosine modifications impede SARS-CoV-2 RNA-dependent RNA transcription

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FIGURE 1.
FIGURE 1.

In vitro assay as used to study SARS-CoV-2 transcription on modified RNA templates. (A) Experimental workflow of kinetics experiments, showing in order binding of RNA template and fluorescent primer, binding of nonstructural proteins, addition of UTP, quenching at time points, then RNA primer and extension product resolved and visualized on Urea-PAGE. (B) Chemical structures of adenosine, N6-methyladenosine, and 2′-O-methyladenosine.

This Article

  1. Published in Advance June 28, 2024, doi: 10.1261/rna.079991.124 RNA 30: 1141-1150

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