rpoS and flhD, two ArcZ targets conserved in Enterobacterales. Alignment of (A) rpoS mRNA sequence and (B) flhD mRNA sequence from Pantoeae ananatis, Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, Photorhabdus laumondii, Xenorhabdus nematophila, Dickeya dadantii, Pectobacterium carotovorum, Serratia marcescens, Proteus mirabilis, Erwinia amylovora, Providencia alcalifaciens, Edwardsiella tarda, Shigella sonnei, Citrobacter freundii, Enterobacter cloacae, and Cronobacter sakazakii (genomes are the same as those used in Fig. 1). Conservation score is plotted below, and on this plot the interaction zone between ArcZ and rpoS/ArcZ and flhD shown in E. coli and E. amylovora is colored red. The interaction of E. coli ArcZ (in red) with rpoS and flhD mRNA and of E. amylovora ArcZ (in green) with flhD mRNA is shown (Mandin and Gottesman 2010; De Lay and Gottesman 2012; Schachterle et al. 2019b). The red squares correspond to ArcZ binding site regions conserved between E. coli and other bacteria. Alignments were performed using MUSCLE (Edgar 2004) and processed with Jalview (Clamp et al. 2004).
