PACRAT: pathogen detection with aptamer-observed cascaded recombinase polymerase amplification–in vitro transcription

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FIGURE 6.
FIGURE 6.

PACRAT is robust to saliva sample matrix. Pre-COVID-19 saliva was added to PACRAT reactions. Bars show end point fluorescence for reactions with SARS-CoV-2 B.1.167 Spike RNA target (left two bars) or without target (right two bars). Individual data points for each triplicate are shown as black circles. Error bars represent SEM, where n = 3.

This Article

  1. Published in Advance April 18, 2024, doi: 10.1261/rna.079891.123 RNA 30: 891-900

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