Multiplexed detection of pathogens by PACRAT. (A) Schematic of multiplexed detection of two RNA targets: AggR gene in E. coli and the spike gene in B.1.167 SARS-CoV-2. Reactions with only E. coli target should only exhibit Broccoli fluorescence, whereas reactions with only the SARS-CoV-2 target should exhibit only Pepper fluorescence. Reactions containing both targets should show dual fluorescence and reactions with no target should show no fluorescence in any channel. (B) Bar graph showing end point fluorescence values for Broccoli and Pepper aptamers in reactions with SARS-CoV-2 RNA target or E. coli RNA target, or both targets or neither. Individual data points for each triplicate are shown as black circles. Error bars represent SEM, where n = 3.
