FIGURE 3.
Limit of detection of PACRAT with SARS-CoV-2 N gene. (A) The limit of detection for PACRAT reactions with SARS-CoV-2 RNA is shown to be 4.5 copies/reaction. Shaded area represents SEM, where n = 3. Transilluminator image shows fluorescence of reactions. (B) (Left) Urea-PAGE gel is stained with SYBR Gold to show RNA product from the end point of reactions with 4.5 copies of SARS-CoV-2 RNA target versus reactions with no target. The gel is staining the expected RNA product at a size of 127 nt. (Right) DFHBI-stained gel, where DFHBI is the cognate dye for the Broccoli aptamer, shows the band that contains the Broccoli aptamer, also at the expected size.
