Snt309 is necessary for the interaction between Prp19C and Mud2, and Snt309 and Cwc15 are important for the interaction of Prp19C with RNAPII. (A) Coomassie gel of the EGTA eluates of Prp19C purified via TAP-tagged Syf1 from wild-type (WT) and the five deletion strains. The levels of copurifying HA-tagged Mud2 and Rpb1 were assessed by western blotting with an antibody directed against HA (αHA) or the antibody 8WG16 recognizing Rpb1, respectively. A strain expressing Mud2-HA only (i.e., lacking Syf1-TAP) served as negative control. (B,C) Exemplary western blot of three independent experiments using αHA (B) and 8WG16 (C) antibodies as described above (left panels) and quantification of the respective bands normalized to the essential Prp19C component Syf1 (right panels).
