Cytosolic RNA binding of the mitochondrial TCA cycle enzyme malate dehydrogenase

Pharmacological perturbation of cellular NAD⁺ levels affects MDH2's RNA binding. (A) Scheme of the salvage synthesis pathway for NAD⁺ showing the steps targeted by NAM and FK866 treatment, respectively. (B) Changes in cellular NAD(H) levels were measured after 48 h of treatment. (C) PNK assay to assess MDH2's RNA binding on alteration of cellular NAD(H) levels. Black arrows indicate the expected molecular mass of mature MDH2. (D) Quantification of RNA binding (PNK signal) of MDH2 after NAM/FK866 treatment. Data are reported as mean ± SD, number of experiments n = 3, individual P-values as calculated by ordinary one-way ANOVA with Tukey's multiple comparison test correction.