MDH2 binds RNA preferentially outside of mitochondria. (A) Experimental scheme. (B) Western blot confirming mitochondrial enrichment. TOM20, an outer mitochondrial protein, was used as a mitochondrial marker; DHX9, a cytosolic protein, was used to assess the purity of the mitochondrial preparation. (C) PNK assay for MDH2 reveals a stronger RNA signal from whole-cell lysates compared to mitochondrially enriched fractions. Black arrows indicate bands near the expected molecular mass of MDH2. (D) Quantification of immunoprecipitated MDH2 (IP-WB) and copurified RNA (IP-PNK). Data are reported as mean ± SD number of experiments, n = 3, individual P-values are indicated according to an unpaired two-tailed t-test.
