Inefficient assembly of CCs on FOXP3 intron 11. (A) Diagram of a CC assembly on pre-mRNA. The splice site motifs are recognized by the U1 snRNP, SF1, and U2AF heterodimers, respectively, forming the CC (created with BioRender). (B) Abundance of U1 snRNP components on F3i11wt-tobra and F3i11pU-tobra (see Materials and Methods for the calculation of abundance). (C) Abundance of SF1 and U2AF heterodimers on F3i11wt-tobra and F3i11pU-tobra. (D) Abundance of U2 snRNP components on F3i11wt-tobra and F3i11pU-tobra. In B–E, all incubations were done in the absence of added ATP, and each bar graph is the median ± interquartile range of four replicates from two independent experiments; (*) P < 0.05, Mann–Whitney test.
