Robo-Therm, a pipeline to RNA thermometer discovery and validation

Using RNArobo to search for potential RNA thermometers similar to the second stem of the agsA thermometer. (A) The entire agsA RNA thermometer secondary structure (Waldminghaus et al. 2007b). (B) A visualization of the map and descriptor. (C) RNArobo search using the second stem (P2 and L2) of the agsA thermometer as a scaffold. Our map and descriptor with the conservation of four uracil nucleotides in a hairpin base paired with the Shine–Dalgarno sequence, and ending with the AUG start codon. “s” denotes single-stranded regions, “h” denotes helical regions in the structure, “N” denotes any nucleotide, and “*” denotes a nucleotide may or may not be present.