A role for SNU66 in maintaining 5′ splice site identity during spliceosome assembly

TABLE 4.

PCR primers used in screening for CRISPR mutations and tracking alleles

Strain Forward primer Reverse primer
snrp-27(M141T) GAGTCGTTACAAAGTGGAGC TTCGCCATGGTCAAATTCCC
snu-66 H765 randomized GTGGACCAGTTATGCCATTC CCACTGAGCACAAGATACGG
Prp8 H1573 randomized CTCTGGTGGTCTCCAACTATC CATAATCTCCCCAACGAAGC
ubc-22 5′ss swap CTCACGGTACGCTGTCATTT GATGTTCAGTATGTGGGCTTCA

This Article

  1. Published in Advance March 5, 2024, doi: 10.1261/rna.079971.124 RNA 30: 695-709

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