A role for SNU66 in maintaining 5′ splice site identity during spliceosome assembly

TABLE 1.

Strains used in this study

Strain name Genotype Notes
JW101 unc-73(e936)I; sup-39(je5)II je5 is U1 snRNA e936 suppressor
SZ118 unc-73(e936) snrp-27(az26)I snrp-27(M141T) e936 suppressor strain
SZ181 unc-73(e936)I
SZ211 snrp-27(az56)I snrp-27(M141T)
SZ326 snu-66(az138)V snu-66(H765S)
SZ370 snu-66(az160)V snu-66(H765G)
SZ371 snu-66(az161)V snu-66(H765L)
SZ372 snu-66(az162)V snu-66(H765C)
SZ427 unc-73(e936)I; snu-66(az160)V snu-66(H765G) e936 suppressor strain
SZ444 ubc-22(az187)X ubc-22 alt 5′ss swap
SZ445 unc-73(e936)I; sup-39(je5)II; snu-66(az160)V sup-39; snu-66(H765G) double e936 suppressor
SZ447 ubc-22(az188)X ubc-22 2 nt intron deletion control
SZ448 snu-66(az160)V; ubc-22(az187)X ubc-22 alt 5′ss swap with snu-66(H765G)
SZ457 snu-66(az160)V; ubc-22(az188)X ubc-22 2 nt intron del. control with snu-66(H765G)

This Article

  1. RNA 30: 695-709