(A) Sequences for the ubc-22 alternative 5′ splicing event. The top sequence is the wt sequence for ubc-22. The middle sequence shows ubc-22 with the AA dinucleotide deletion, which is a control for the 5′ swap experiment below. The bottom sequence is ubc-22 with the 5′ss sequences swapped and the AA dinucleotide deletion. The blue sequence and black arrow represent the wt upstream splice site, while the yellow sequence and white arrow show the wt downstream sequence. The gray AA are the two nucleotides that were deleted. (B) Cy3 RT-PCR products separated on a 6% denaturing polyacrylamide gel for the alternatively spliced region of ubc-22. The genotype [ubc-22 mutant status and snu-66(H765G) mutant status] is shown above each lane. Quantitation is shown below the gel image.
