TABLE 1.
Yields, purities, and endotoxin activity levels of individual BioRNA/miRNAs using human leucyl or glycyl tRNA fused hsa-pre-miR-34a carrier, and isolated by anion exchange FPLC methods
| microRNA | Total RNA (mg/250 mL fermentation) | BioRNALeu | Total RNA (mg/250 mL fermentation) | BioRNAGly | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Yield of pure BioRNA | Purity (%, by HPLC) | Pure BioRNA endotoxin activity (EU/µg RNA) | Yield of pure BioRNA | Purity (%, by HPLC) | Pure BioRNA endotoxin activity (EU/µg RNA) | |||||
| mg/250 mL fermentation | % of Total RNA | mg/250 mL fermentation | % of Total RNA | |||||||
| miR-126b-3p | 11.3 | 6.01 | 53.2 | 99.7 | 1.51 | 23.2 | 10.1 | 43.5 | 99.1 | 4.20 |
| miR-133a-3p | 13.8 | 6.65 | 48.2 | 98.2 | 3.00 | 18.2 | 8.16 | 44.8 | 99.4 | 3.90 |
| miR-200b-3p | 19.0 | 7.80 | 41.1 | 98.7 | 0.18 | 24.1 | 9.20 | 38.2 | 99.6 | 3.86 |
| miR-7-5p | 9.00 | 2.63 | 29.2 | 99.5 | 5.88 | 16.0 | 5.00 | 31.3 | 98.7 | 0.68 |
| miR-143-3p | 14.2 | 6.70 | 47.2 | 98.8 | 4.70 | 30.9 | 14.3 | 46.3 | 98.9 | 0.28 |
| miR-206-3p | 10.5 | 4.77 | 45.4 | 97.7 | 2.30 | 17.8 | 4.65 | 26.1 | 97.8 | 2.26 |
| miR-375-3p | 14.3 | 11.2 | 78.3 | 98.3 | 4.30 | 26.5 | 11.9 | 44.9 | 99.1 | 0.46 |
| miR-497-5p | 18.0 | 5.10 | 28.3 | 97.6 | 2.80 | 22.3 | 7.75 | 34.8 | 99.0 | 1.00 |
| miR-125b-5p | 37.8 | 14.1 | 37.3 | 97.5 | 0.75 | 20.2 | 9.29 | 46.0 | 98.6 | 0.80 |
| let-7a-5p | 19.3 | 10.9 | 56.5 | 99.5 | 0.60 | 28.4 | 14.7 | 51.8 | 99.1 | 1.20 |
| let-7b-5p | 17.2 | 10.5 | 61.0 | 98.5 | 0.98 | 31.4 | 12.6 | 40.1 | 99.2 | 0.70 |
| let-7c-5p | 16.2 | 4.13 | 25.5 | 99.1 | 1.20 | 13.8 | 6.78 | 49.1 | 98.8 | 0.70 |
| let-7d-5p | 18.1 | 9.66 | 53.4 | 98.6 | 3.00 | 15.6 | 8.68 | 55.6 | 99.1 | 0.58 |
| let-7e-5p | 14.7 | 6.99 | 47.6 | 98.4 | 2.50 | 17.3 | 6.63 | 38.3 | 99.0 | 1.00 |
| let-7f-5p | 22.8 | 11.4 | 50.0 | 98.8 | 1.25 | 14.4 | 6.86 | 47.6 | 99.1 | 0.30 |
| let-7g-5p | 18.1 | 8.91 | 49.2 | 99.5 | 0.68 | 19.1 | 9.13 | 47.8 | 99.0 | 0.54 |
| miR-101-3p | 19.8 | 11.6 | 58.6 | 99.1 | 2.90 | 16.5 | 5.87 | 35.6 | 99.0 | 2.45 |
| miR-195-5p | 20.4 | 9.70 | 47.5 | 99.8 | 5.00 | 17.4 | 6.64 | 38.2 | 99.2 | 2.10 |
| miR-370-3p | 16.4 | 10.4 | 63.4 | 99.4 | 1.90 | 26.1 | 9.00 | 34.5 | 99.0 | 4.90 |
| miR-495-3p | 20.7 | 7.54 | 36.4 | 97.8 | 1.08 | 12.9 | 1.68 | 13.0 | 97.1 | 5.00 |
| miR-519a-5p | 21.8 | 8.92 | 40.9 | 97.6 | 2.29 | 16.5 | 4.75 | 28.8 | 99.0 | 4.49 |
| miR-29a-3p | 18.2 | 10.0 | 54.9 | 97.6 | 1.97 | 21.9 | 7.97 | 36.4 | 97.5 | 1.22 |
| miR-99-5p | 16.7 | 6.76 | 40.5 | 99.8 | 6.80 | 23.9 | 11.8 | 49.4 | 99.1 | 3.18 |
| miR-100-5p | 18.8 | 7.85 | 41.8 | 99.5 | 8.64 | 18.9 | 8.14 | 43.1 | 98.9 | 3.34 |
| miR-146a-5p | 16.2 | 8.20 | 50.6 | 99.5 | 5.29 | 19.3 | 7.96 | 41.2 | 99.0 | 4.25 |
| miR-146b-5p | 17.8 | 7.78 | 43.7 | 99.1 | 4.82 | 16.2 | 5.72 | 35.3 | 99.2 | 5.00 |
| Mean ± SD | 17.7 ± 5.3 | 8.32 ± 2.6 | 47.3 ± 11.5 | 98.8 ± 0.8 | 2.94 ± 2.2 | 20.3 ± 5.2NS | 8.28 ± 3.0NS | 40.1 ± 9.1NS | 98.9 ± 0.6NS | 2.25 ± 1.7NS |
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The shaded BioRNAs were reported recently and included for comparison.
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(FPLC) fast protein liquid chromatography, (HPLC) high-performance liquid chromatography.
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NSNot statistically significant, compared with BioRNALeu (one-way ANOVA with Bonferroni post hoc tests).
