Screen for antiproliferative effects of 48 novel, highly purified BioRNAs against human NSCLC cells. Cell viability of A549 (A) and H1975 (B) cells was reduced to various degrees by BioRNAGly (blue) and BioRNALeu (red) based miRNAs (15 nM) as compared with control BioRNA or vehicle as well as untreated cells. Cell viability values were determined by CellTiter-Glo assay at 72 h post-transfection and normalized to respective control RNAs (100%). Values are mean ± SD (N = 4 biological replicates per group). #Several BioRNAs published recently were included for comparison. Overall, the effects on NSCLC cell viability were comparable between the paired BioRNAGly- and BioRNALeu-based miRNAs with putative cell line-specific differences. BioRNA/miR-7-5p used for further studies are underlined. (*) P < 0.05, as compared to respective control RNA (one-way ANOVA with Bonferroni post hoc tests).
