Novel RNA molecular bioengineering technology efficiently produces functional miRNA agents

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 2.
FIGURE 2.

Purification of recombinant miRNA molecules. (A) Representative FPLC-UV trace during the purification of the model BioRNAGly/miR-7-5p with a highlighted peak for target fraction collection. (B) Urea–PAGE analysis of BioRNAGly/miR-7-5p fractions. (C) HPLC determination of the purity (99.6%) of final BioRNAGly/miR-7-5p product. (D, E) Urea–PAGE analyses depicting FPLC-purified BioRNAGly/miRNAs and BioRNALeu/miRNAs, respectively (50 ng RNA/lane). Purification of BioRNALeu/miR-7-5p, -126b-3p, -133a-3p, and -200b-3p has been published recently (Li et al. 2021).

This Article

  1. Published in Advance March 1, 2024, doi: 10.1261/rna.079904.123 RNA 30: 680-694

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA