A comparative analysis of peptide-delivered antisense antibiotics using diverse nucleotide mimics

TABLE 2.

Mass-spectrometry-based detection of antisense oligomer-peptide fragments in Salmonella periplasm and cytoplasm 15 min post-treatment

KFF-ASO Periplasm Fragments detected
(KFF)3K-PNA ✓ PNA (+KFF)
(KFF)3KK(N3)-PMO ✓ PMO
(KFF)3KK(N3)-LNA ND -
(KFF)3KK(N3)-RNA-F ND -
(KFF)3KK(N3) RNA-MOE ND -
(KFF)3KK(N3)RNA-OMe ND -

  • (ASO) Antisense oligomer, (PNA) peptide nucleic acid, (PMO) phosphorodiamidate morpholino, (LNA) 2′–4′-locked RNA, (RNA-F) 2′-fluorinated RNA, (RNA-MOE) 2′-methoxyethylated RNA, (RNA-OMe) 2′-methylated RNA, (ND) not detected.

This Article

  1. Published in Advance February 27, 2024, doi: 10.1261/rna.079969.124 RNA 30: 624-643

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