Western blot analysis of AcpP levels upon KFF-ASO treatment of Salmonella. (A) Western blot analysis of Salmonella strain SL1344 expressing AcpP::3xFLAG treated with KFF-PNA or KFF-PMO, each at 5 µM, for 30, 60, and 120 min. (B) Western blot analysis of Salmonella treated with all acpP-targeting KFF-ASO constructs at 5 µM for 120 min. (A,B) A scrambled KFF-PNA conjugate (PNA scr) and mock-treated (water) samples served as negative controls. Protein lysates were separated on 12% SDS-PAA gels, and proteins were blotted onto nitrocellulose membranes. Membranes were probed with a FLAG-specific antibody to detect the AcpP::3xFLAG fusion protein. GroEL was used as a loading control. The experiments were performed two times and exemplary images are shown.
