FIGURE 3.
ASO:RNA duplex binding studies and in vitro translation assays. (A) Melting curves of acpP RNA–ASO complexes at equimolar concentrations. The target RNA used in this experiment is 10 nt long. (B) The capacity of the ASOs to inhibit translation of an acpP::gfp reporter transcript in vitro was analyzed at 5:1, 2.5:1, 1.25:1, and 0.6:1 molar ratios of ASO:RNA. Graphs show Image J quantitation of the GFP fusion protein in a western blot analysis of two independent experiments (replicates are color-coded in red and blue). Protein expression levels are shown relative to the water control. The corresponding western blots are shown in Supplemental Figure S5.
