A comparative analysis of peptide-delivered antisense antibiotics using diverse nucleotide mimics

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FIGURE 2.
FIGURE 2.

Electrophoretic mobility shift assay. Retardation of electrophoretic mobility of a 5′ Cy5-labeled acpP target RNA (40 nt) dependent on ASO hybridization. Different ASO modalities were tested at 2.5:1, 1.25:1, 0.6:1, and 0.3:1 molar ratios of ASO:RNA as indicated. Yeast tRNA (10 µg) was spiked in samples labeled with “+.” Samples were separated on 15% native PAA gels, and one representative example of two independent experiments is shown.

This Article

  1. Published in Advance February 27, 2024, doi: 10.1261/rna.079969.124 RNA 30: 624-643

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