TABLE 1.
High-throughput methods for site-specific mapping of Nm sites in RNA
| Method | Approach | Stoichiometry | Applications |
|---|---|---|---|
| RiboMeth-seq (Marchand et al. 2016) | Alkaline hydrolysis | Absolute | rRNA, tRNA, snRNA |
| RibOxi-seq (Zhu et al. 2017) | Periodate oxidation | No | rRNA |
| Nm-seq (Dai et al. 2017) | Periodate oxidation | No | rRNA, mRNA, miRNA, piRNA |
| 2OMe-seq (Incarnato et al. 2017) | RT at low [dNTP] | Relative | rRNA |
| MeTH-seq (Bartoli et al. 2018) | RT at low [Mg] | No | mRNA |
| Nm-Mut-seq (Chen et al. 2023) | Engineered RTase | Relative | rRNA, mRNA |
| NJU-seq (Tang et al. 2024) | MgR cleavage | Relative | rRNA, mRNA |
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(MgR) Mycoplasma genitalium RNase R; (RT) reverse transcription; (dNTP) deoxynucleotide triphosphate concentration; (Mg) magnesium concentration; (RTase) reverse transcriptase.
