Red-m1A-seq detected the known and new m1A sites in human tRNA and rRNA. (A) Heatmap showing the average mutation rates detected on the m1A58 site across 50 tRNA genes using red-m1A-seq, based on data from three technical replicates in HepG2 cells. (B) Heatmap showing the average mutation rates detected on the m1A9 site across 15 tRNA genes using red-m1A-seq, based on data from three technical replicates in HepG2 cells. (C) Mutation rate at position 72 on mt-tRNAAsn-GTT was measured using red-m1A-seq, based on data from three technical replicates in HepG2 cells. Error bars represent SD. (D) Table showing the information of m1A sites identified in cytosolic rRNA by red-m1A-seq. Newly identified m1A sites are highlighted in yellow rectangle. (E) Secondary structure of mt-tRNAAsn with its reported modifications. (m2G) N2-methylguanosine, (D) dihydrouridine, (m5U) 5-methyluridine, (Ψ) pseudouridine, (Q) queuosine, (t6A) N6-threonylcarbamoyladenosine.
