Challenges to mapping and defining m⁶A function in viral RNA

Recent strategies in m⁶A mapping and quantification that could be applied to viral infection studies. (A) “Molecular recording” techniques in which m⁶A readers are fused to RNA editing enzymes can address issues with input requirements, quantitative measurement, and isoform (when paired with cDNA long-read sequencing). (B) Protection-based sequencing techniques selective for m⁶A can directly map m⁶A at single-nucleotide resolution with absolute quantification. (C) Measuring m⁶A using long-read direct RNA sequencing can identify individual m⁶A RNA profiles across a population of transcripts at high resolution.